Objective: The aim of this research was to compare prosocial personality, psychological reactance, perceived social support, and defense styles in parents of children with physical and mobility impairment and healthy children in Isfahan, Iran. Method: The present research adopted a descriptive (ex post facto) design. The target population comprised the parents of children with physical and mobility impairment in Isfahan. A sample of 30 parents of children with the noted impairment and 30 parents of healthy children was recruited via convenience sampling. The measures used included Penner’s Prosocial Personality Battery, Zimet’s Multidimensional Scale of Perceived Social Support, Hong and Page’s Psychosocial Reactance Scale, and Andrews’ Defense Style Questionnaire. Data were analyzed using MANOVA. Results: Results demonstrated a significant difference between the two groups in terms of dimensions of support from the family (p≤0.01), support from friends (p≤0.05), directional empathy (p≤0.01) and prosocial personality (p≤0.01). The level of support from the family, directional empathy, and prosocial personality was higher in parents of children with physical and mobility impairment compared to the parents of healthy children. However, the level of support from friends was higher for parents of healthy children than those of children with the noted impairment. Conclusion: The results of this study confirmed the importance of perceived social support and prosocial personality in parents of children with physical and mobility impairment. It is necessary to include these factors while developing health interventions for these parents.